Gastric cancer, Q fever, Legionnaires’ illness, whooping cough–through the infectious microorganism that triggers these serious ailments are different, all of them utilize the identical molecular machinery to contaminate human cells.
Microorganism use this machinery, known as a Type IV secretion system (T4SS), to inject poisonous molecules into cells and likewise to unfold genes for antibiotic immunity to fellow microorganism. Researchers at Caltech have revealed the 3D molecular structure of the T4SS from the human pathogen Legionella pneumophila with unique details. This could sooner or later allow the development of targeted antibiotics for the ailments mentioned above.
The task was done in the laboratory of Grant Jensen, professor of biophysics and biology and Howard Hughes Medical Institute researcher, in collaboration with the laboratory of Joseph Vogel on the Washington University Faculty of Medicine in St. Louis (WUSTL). A paper describing the analysis appeared online on April 22 in the journal Nature Microbiology.
There are nine several types of bacterial issue techniques, Type IV being mostly the most elaborate and versatile. A T4SS can carry all kinds of poisonous molecules–as much as 300 without delay–from a bacterium into its cellular sufferer, hijacking mobile capabilities and bewildering the cell’s defenses.
In 2017, Caltech scholar Debnath Ghosal and his associates used a method known as electron cryotomography to reveal, for the first time, the low-resolution structure of the T4SS in Legionella, the microorganism that causes Legionnaires’ illness, an extreme and often deadly type of pneumonia.
Ghosal, alongside Kwang-Cheol Jeong of WUSTL and their associates, has now made an in-depth structural model of this powerful multi-element machine. The group additionally made perturbations to the bacterium’s genes to review mutant variations of the T4SS, unveiling how this complex machine organizes and assembles.